Yang, Kang, and Choi: The role of the acoustic radiation force in color Doppler twinkling artifacts

Abstract

Purpose:

The aim of this experimental study was to evaluate whether the acoustic radiation force (ARF) is a potential source of twinkling artifacts in color Doppler images.

Methods:

Color Doppler images were obtained using a clinical ultrasonic scanner (Voluson e, GE Healthcare) for a high contrast (+15 dB) circular scattering phantom at pulse repetition frequencies (PRFs) ranging from 0.1 to 13 kHz. Ultrasound transmissions resulting in ARF were measured using a hydrophone at the various PRFs considered. The influence of ARF on the appearance of twinkling colors was examined via the common parameter PRF. This methodology is based on the fact that alternating positive and negative Doppler shifts induced by the ARF are centered at a PRF twice the maximum Doppler frequency on the color scale bar, whereas the twinkling color aliasing is expected to remain similar regardless of PRF.

Results:

Color twinkling artifacts were observed to be most conspicuous at the lowest PRF of 0.1 kHz. The extent of twinkling rapidly decreased as the PRF increased, eventually disappearing when the PRF ≥0.6 kHz. The measured ultrasound transmissions, however, were found to be insensitive to the PRF, and therefore it can be inferred that the PRF was insensitive to the ARF.

Conclusion:

Based on our experimental observations, the ARF may not be a source of color Doppler twinkling artifacts.

Introduction

When color Doppler images are obtained for static scattering targets, twinkling colors may appear due to the presence of turbulent flows [1,2]. This effect is often referred to as the presence of twinkling artifacts. The mechanisms underlying color Doppler twinkling artifacts are not clearly understood yet, even though the twinkling is known to be target-dependent and is caused by noises that arise from ultrasonic scanners [3]. Recently, the acoustic radiation force (ARF) has been proposed as the potential source of color Doppler twinkling artifacts, but this has not yet been experimentally verified [4].
The ultrasound used to construct color Doppler images produces an ARF that pushes targets, which are also known as scattering particles. The resulting target movement induces a frequency shift known as the Doppler shift in the ultrasound that is received. In such a case, the target movement resulting from the ARF is repeated at the frequency of a given pulse repetition frequency (PRF). This implies that Doppler shifts resulting from the ARF are narrowbanded and are centered at the PRF.
Users in the color Doppler mode cannot readily control the ARF, making it difficult to clinically examine the effects of radiation force on twinkling artifacts. However, the PRF, which reflects the frequency of the ARF that is exerted on targets, can be set by users. Careful observation of twinkling colors in relation to the PRF may provide insights as to whether color Doppler twinkling artifacts are associated with the ARF. This study aimed to experimentally investigate the significance of the ARF as a potential source of twinkling artifacts in color Doppler images.

Materials and Methods

Twinkling images were obtained at varying PRFs on a highcontrast circular static target containing highly echogenic scattering particles. In order to examine the influence of the PRF on the ARF, the transmitting ultrasounds produced by the ultrasonic scanner were measured in a water tank using a hydrophone. Based on these experimental results, the role of the ARF on color Doppler twinkling artifacts was assessed.

Color Doppler Imaging of a Scattering Contrast Target

A color Doppler image was obtained of a circular scattering target using a GE ultrasound scanner (Voluson e, GE Healthcare, Oberösterreich, Austria) with a linear probe (12L-RS, 3-12 MHz). The scattering target was taken from the +15 dB circular contrast target of a commercial ultrasound standard quality assurance phantom (Model 551 small parts phantom, ATS Laboratories Inc., Bridgeport, CT, USA). The diameter of the target was 6 mm. The phantom was placed on a vibration-free supporter (RFB, NTR Systems, Seattle, WA, USA) to isolate the phantom from external vibrations. Ultrasonic coupling gel was applied to the upper surface of the phantom before scanning. The focal zone of the probe was set at the center of the target [5-8]. Color Doppler images were acquired under the default settings of the control parameters except for the PRF (e.g., Doppler frequency low, Doppler gain 0, and wall motion filter high). The extent of the twinkling was quantified by the number of color pixels within the target [9,10]. The color pixel number was counted using MatLab (MATLAB R2009a, MathWorks Inc., Natick, MA, USA).

Measurements of Transmitted Ultrasonic Pulses

The ARF that can induce a target to vibrate at the PRF is produced by each transmitted ultrasonic pulse that irradiates a target when the ultrasonic scanner is operated in the color Doppler mode. The ARF is estimated from the transmitted ultrasonic pulse and the acoustic properties of the medium through which the ultrasound pulse propagates. For a given imaging target, the ARF is approximately derived from the transmitted ultrasonic pulse measured in a reference medium. The transmitted pulse was measured in a water tank using a needle hydrophone (TNU001A, NTR Systems) at nominal PRFs ranging from 0.1 kHz to higher values (Fig. 1). The measured transmitted pulses were stored on a digital scope (CS122G1, GaGe, Dynamic Signals LLC, Lockport, IL, USA). The pulse intensity integral of each transmitting ultrasonic pulse was calculated from the measured waveform, which is closely related to the ARF [11]. The recordings were repeated 12 times in order to examine the variability of the pressure and the intensity of transmitted ultrasonic pulses.

Results

Typical transmitted ultrasonic pulses produced by the clinical ultrasonic scanner operated in color Doppler mode are shown in Fig. 2, measured in a water tank with nominal PRFs set to 0.1 kHz, 0.3 kHz, 0.6 kHz, 0.9 kHz, 2.4 kHz, and 13 kHz. These ultrasonic pulses lasted for three to four cycles at a center frequency of approximately 5 MHz and had negative peak pressures about twice the corresponding positive peak pressures. The measured waveforms, regardless of the PRF, were found to be almost identical.
As expected, the mean values of the peak pressures and the pulse intensity integral remained virtually unchanged (within 3%) as the PRF varied from 0.1 to 13 kHz (Fig. 3). The standard deviations were found to be insignificant, indicating that the transmitted ultrasonic pulses produced for color Doppler imaging were highly stable. The PRF was not observed to have an effect on transmitted ultrasonic pulses, which suggests that the PRF also does not affect the magnitude of the ARF produced by the transmitted ultrasonic pulse that irradiates the target.
Typical color Doppler images (Fig. 4) were obtained for the circular +15 dB scattering contrast target at the nominal PRF values of 0.1, 0.3, and 0.6 kHz, over for a period extending up to 14 seconds from the onset of image acquisition. Less twinkling appeared as the PRF increased and twinkling artifacts could not be seen at PRF settings of 0.6 kHz or higher in color Doppler images (Fig. 4). The twinkling colors changed randomly over time, which can be attributed to random Doppler noises. This change is illustrated by the time history of the color pixel number within the target, which was displayed for 12 seconds at the nominal PRF settings of 0.1, 0.3, and 0.6 kHz (Fig. 5A). The temporal mean values of the extent of color twinkling were highest at the lowest PRF (0.1 kHz) and exponentially decreased as the PRF increased. A similar trend was found in the temporal variability of the twinkling plotted against the PRF (Fig. 5B).

Discussion

The twinkling artifact in color Doppler images can be easily seen at the rear of strong reflective targets, including renal stones [5,6,12-14] and guided needle punctures [15], as well as around or inside scattering soft tissue lesions such as calcified pancreatitis [15] and liver hyperechoic masses [15,16]. This suggests that color Doppler twinkling artifacts are associated with the echogenicity of targets and the acoustic impedance mismatch between the targets and the background. The impedance mismatch may cause a significant ultrasonic wave reflection at the interface of the target, possibly resulting in a radiation force directed toward the target.
If it were the case that a transmitted ultrasonic pulse produces the ARF exerted on a target, causing it to vibrate, the resulting alternating Doppler shifts displayed on the image as color twinkling would be narrow-banded. The spectra of the Doppler signals would be centered at the PRF, because the ARF is repeatedly exerted on the target to induce vibration at the PRF. The narrow band, however, is located far beyond the range of the color scale bar (from -PRF/2 to +PRF/2), and thus results in color (spectral) aliasing. The aliasing is displayed on the image with twinkling colors. If the ARF induces the aliasing, the resulting twinkling color would be expected to appear at all values of the PRF. In the present study, however, the twinkling artifacts appeared when the PRF was either 0.1 or 0.3 kHz, but completely disappeared when the PRF exceeded 0.6 kHz (Figs. 4, 5). This corresponds to previous observations that color Doppler twinkling artifacts highly dependent on the PRF of the ultrasound transmission, as reported by Kamaya et al. [10].
It should be noted that contradictory findings have been reported regarding the relationship between twinkling artifacts and the PRF. One group of researchers [17,18] has reported that more twinkling appeared at higher PRFs, whereas other investigators [3,7,9] have claimed the opposite results. Such contradictory observations can probably be attributed to differences in the targets and ultrasonic scanners used in each study.
In the present study, the measured transmitted ultrasonic pulses remained virtually unchanged while the PRF varied from 0.1 to 13 kHz (Figs. 2, 3). This suggests that the PRF does not affect the magnitude of the ARF induced by the transmitted ultrasonic pulse that irradiates the target, as the ARF is proportional to the pulse intensity integral. Linking these two observations (first, that the ARF was insensitive to the PRF and second, that the extent of color Doppler twinkling was highly affected to the PRF) leads to the deduction that the ARF is unlikely to be associated with color Doppler twinkling artifacts. This deduction is valid for the present experimental conditions performed on a static scattering contrast target with a clinical ultrasonic scanner. Further verification is required on other clinical scanners and in other conditions.
In summary, the color Doppler twinkling that appeared in a circular scattering target was observed to depend on the PRF setting. The PRF, however, was found to be insensitive to the ARF resulting from each transmitted ultrasonic pulse that irradiated the imaging target. No evidence was found of ARF-induced twinkling aliasing, which, if occurred, was expected to appear at all values of PRF. These results suggest that the ARF is unlikely to be associated with color Doppler twinkling artifacts, and therefore, that the ARF cannot be considered as a potential source for color twinkling artifacts. This conclusion is valid under the present experimental conditions for color Doppler imaging, and further verification is required with regard to other clinical conditions.

Conflicts of Interest

No potential conflict of interest relevant to this article was reported.

Acknowledgements

This research was supported by the 2014 scientific promotion program funded by Jeju National University, Republic of Korea.

References

1. Rubens DJ, Bhatt S, Nedelka S, Cullinan J. Doppler artifacts and pitfalls. Radiol Clin North Am 2006;44:805–835.
crossref pmid
2. Rahmouni A, Bargoin R, Herment A, Bargoin N, Vasile N. Color Doppler twinkling artifact in hyperechoic regions. Radiology 1996;199:269–271.
crossref pmid
3. Yang JH, Kang GS, Choi MJ. Sonographic aspects of twinkling artifacts. J Acoust Soc Korea 2010;29(2 Suppl):189–192.

4. Behnam H, Hajjam A, Rakhshan H. Modeling twinkling artifact in sonography. In: 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE); 2010 Jun 18-20; Chengdu, China.
crossref
5. Lee JY, Kim SH, Cho JY, Han D. Color and power Doppler twinkling artifacts from urinary stones: clinical observations and phantom studies. AJR Am J Roentgenol 2001;176:1441–1445.
crossref pmid
6. Mitterberger M, Aigner F, Pallwein L, Pinggera GM, Neururer R, Rehder P, et al. Sonographic detection of renal and ureteral stones. Value of the twinkling sign. Int Braz J Urol 2009;35:532–539.
crossref pmid pdf
7. Tsao TF, Kang RJ, Gueng MK, Tyan YS, Lin YC, Lee SK. Color Doppler twinkling artifact and clinical use. J Med Ultrasound 2009;17:157–166.
crossref
8. Shabana W, Bude RO, Rubin JM. Comparison between color Doppler twinkling artifact and acoustic shadowing for renal calculus detection: an in vitro study. Ultrasound Med Biol 2009;35:339–350.
crossref pmid
9. Wang M, Li J, Xiao J, Shi D, Zhang K. Systematic analysis of factors related to display of the twinkling artifact by a phantom: an optimized investigation. J Ultrasound Med 2011;30:1449–1457.
crossref pmid
10. Kamaya A, Tuthill T, Rubin JM. Twinkling artifact on color Doppler sonography: dependence on machine parameters and underlying cause. AJR Am J Roentgenol 2003;180:215–222.
crossref pmid
11. Palmeri ML, Sharma AC, Bouchard RR, Nightingale RW, Nightingale KR. A finite-element method model of soft tissue response to impulsive acoustic radiation force. IEEE Trans Ultrason Ferroelectr Freq Control 2005;52:1699–1712.
crossref pmid pmc
12. Sohn CH, Lee JH, Cho KS. Twinkling artifact on color Doppler ultrasound: comparison among various composition of stones and various central frequencies of transducers. J Korean Soc Med Ultrasound 2002;21:213–218.

13. Chelfouh N, Grenier N, Higueret D, Trillaud H, Levantal O, Pariente JL, et al. Characterization of urinary calculi: in vitro study of "twinkling artifact" revealed by color-flow sonography. AJR Am J Roentgenol 1998;171:1055–1060.
crossref pmid
14. Alan C, Kocoglu H, Kosar S, Karatag O, Ersay AR, Erhan A. Role of twinkling artifact in characterization of urinary calculi. Actas Urol Esp 2011;35:396–402.
crossref pmid
15. Kim HC, Yang DM, Jin W, Ryu JK, Shin HC. Color Doppler twinkling artifacts in various conditions during abdominal and pelvic sonography. J Ultrasound Med 2010;29:621–632.
crossref pmid
16. Mo GQ, Liu XM. Ultrasonographic findings at the resected area after hepatectomy. Hepatobiliary Pancreat Dis Int 2006;5:401–405.
pmid
17. Girish G, Caoili EM, Pandya A, Dong Q, Franz MG, Morag Y, et al. Usefulness of the twinkling artifact in identifying implanted mesh after inguinal hernia repair. J Ultrasound Med 2011;30:1059–1065.
crossref pmid
18. Tchelepi H, Ralls PW. Color comet-tail artifact: clinical applications. AJR Am J Roentgenol 2009;192:11–18.
crossref pmid

A schematic experimental setup for recording transmitted ultrasonic pulses generated by a clinical ultrasonic scanner (Voluson e, GE Healthcare).

Waveforms were measured in a water tank using a needle hydrophone (TNU001A, NTR Systems) and stored on a digital scope (CS122G1, GaGe, Dynamic Signals LLC).
usg-14065-f1.gif
Fig. 1.

Typical transmitted ultrasonic pulses produced by an ultrasonic scanner with a linear probe operated in color Doppler mode.

The waveforms were measured in a water tank using a needle hydrophone at the nominal pulse repetition frequency values of 0.1 kHz, 0.3 kHz, 0.6 kHz, 0.9 kHz, 2.4 kHz, and 13 kHz.
usg-14065-f2.gif
Fig. 2.

Acoustic output against the pulse repetition frequency (PRF).

The peak magnitudes (A) and the pulse intensity integrals (B) of the transmitted ultrasonic pulses were measured at varying PRFs, and the results suggest that the acoustic radiation force of the ultrasound transmission remained almost the same regardless of the PRF. The mean and standard deviation were obtained from 12 repeated recordings.
usg-14065-f3.gif
Fig. 3.

Typical color Doppler images.

The images were obtained for a circular +15 dB contrast scattering target at the pulse repetition frequencies (PRFs) of 0.1, 0.3, and 0.6 kHz, over up to 14 seconds from the onset of image acquisition, showing that the extent of color twinkling rapidly reduced as the PRF increased.
usg-14065-f4.gif
Fig. 4.

Color pixel number (CPN) against pulse repetition frequency (PRF).

The CPNs within the targets of color Doppler images were obtained for a circular +15 dB scattering target at the nominal PRFs of 0.1, 0.3, and 0.6 kHz. Their temporal variation over 12 seconds (A), and their temporal mean and standard deviation against the PRF (B) demonstrate that the mean values of CPN were dramatically reduced when the PRF was increased.
usg-14065-f5.gif
Fig. 5.
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